Pregnancy was Obtained with Micro Elosi with Elonge Spermatid from Semen with Stem Cell Therapy without TESE!
Significance
Azoospermia is an important problem for some male cases in society. The currently accepted view is that testicular sperm extraction is the most suitable solution for cases where mature sperm cannot be detected in semen.
In recent times, the increasing rate of unsolvable TESE or micro TESE has not been resolved in an acceptable way by repeated TESE.
In addition to the publications stating that the multiple and repetitive TESE approach called Salvage gradually destroys the in vivo tissue growth factors called extracellular matrix in the microenvironment of the cell producing chambers, the success of microenvironmental supports to be added to the correct gonadotropin treatment is increasing compared to TESE operations.
In this article, we present the IVF pregnancy achieved by micro elosi using elonge series spermatid produced from Sertoli cell only table by correct gonadotropin therapy as well as phytotherapeutic supplements after two unsuccessful micro TESE, which was treated with these principles, as the first case of noninvasive pregnancy without TESE in the world.
We hope that this method will be an important milestone that will start the era of biological fatherhood in all azoospermic cases waiting for a baby and we are honoured to contribute to the other steps that need to be taken to achieve healthier generations.
Keywords: Human, Elonge Spermatid, Elosi, Azoospermia, Male Infertility, Detailed Microscopic Sperm Precursor Cell Analysis System
Summary
The solution approaches in the treatment of male infertility, which started with microinjection of sperm into the oocyte with IVF treatment, have achieved limited success with elongated spermatids obtained by micro TESE, and even more recently, news of babies born with round spermatids have just been shared.
The main feature of these methods was that these precursor sperm cells could only be extracted and used with an invasive method such as micro TESE.
What makes our study different is that a healthy pregnancy was achieved by using the elosi procedure, which was performed noninvasively, that is, by selecting and using elongated spermatids obtained in a simple semen sampling examination based on some criteria.
Another detail is that the patient had undergone two previous unsuccessful micro TESE operations and that this procedure was performed with the elonge spermatid selected from the cell series obtained in the last stage by monitoring the progression with quarterly control periods from the very beginning with the detailed sperm precursor cell analysis system and the benefit provided by the phytotherapy added with the gonadotropin treatment starting from the Sertoli cell only table of the testicular pathology. Therefore, a procedure to be followed in reaching a result with treatment is also described instead of calling the patients to the TESE operation again by saying that the regressed system cannot recover.
In addition to the physical morphological characteristics in the selection of the spermatid, attention was paid to the genetic quality infrastructure and the common genetic quality integrity in the embryo.
In trials prior to this method, the success rate of the first embryo formation was higher in all cases than in round spermatids. In addition to this success, we had many cases who had a baby with ICSI by providing normal sperm output despite TESE. In addition to the fact that the normal natural sperm output rate of those who never had TESE was considerably higher than those who had TESE, pregnancy and birth naturally in azoospermic patients have been realised several times.
Our two proven cases of natural pregnancy in the spermatid stage without any spermatozoa have led us to investigate the quality of some spermatids in the research phase of the research phase of good quality samples.
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Now, it is time to roll up our sleeves and correct the mistakes of the past. Now, our voice is louder when we say that TESE should not be performed without the necessary examination methods and treatment methods.
We declare again that we are open to all kinds of information sharing on this subject.
So, How Does Our System Work?
First of all, we want the patient to complete all missing documents related to azoospermia. Hormones, detailed analysis of precursor sperm cells, genetic karyotype and Y chromosome deletion, scrotal, i.e. testicular doppler ultrasonography and transrectal pelvic ultrasonography, investigation of post-testicular conduction channels, determination of inhibin B reserve, biochemical additional tests in necessary cases, serological virological tests and pituitary MRI in some cases and cystic fibrosis and FMF gene analysis in some cases. Information about this is provided on the contact page of our website.
When the files are created, we upload our results to the patient information bank on our website. After that, we inform our patient about the interpretations of all the results, we reveal the risk analysis in terms of TESE and we start the treatment with informed consent. In order to obtain this, we issue an official drug report.
During the follow-ups, controls are performed with a 5-way hormone profile and detailed precursor sperm cell analysis. The basis of the three-month follow-up is related to the renewal process of spermatogenesis. During the follow-ups, it is hoped that the findings in the blood are clear in terms of response criteria and the findings in the semen are clear in terms of response criteria. However, there are sometimes cases of low responsiveness. These may be some resistances experienced in the transition to the next stage of the progress in cell levels from time to time, or if the response in the testis is good, but the semen cannot reflect the good response due to obstruction, further research is required. For example, the blood response may give a good response that is much better than expected, but the semen may not reflect this. In that case, the post-testicular pathways should be investigated more professionally because they do not reflect the outflow response as it really is. A specialised urologist and radiologist are contacted. In general, patients think that examinations in other centres are sufficient. However, under the control of our own specialist, we have repeatedly come across incomplete and erroneous reports on this subject. For example, a report can be received as if everything is normal while there is a vasa obstruction in the post-testicular pathways. In that case, our follow-up criteria will continue to be based on blood, since the semen sample will have a weak effect on follow-up.
In addition, Doppler control of patients receiving gonadotropin hormone treatments is recommended by us once a year. Tumour development is higher in patients with delayed surgery for undescended testis compared to other cases. Doppler also provides information for the clinician for regions with better micro TESE chances by describing better vascularised regions region by region in the reporting. This method, which aims to reduce the risk and increase the chance before TESE, should be a radiological application procedure that should be applied everywhere.
After the consent, the SSI report is issued, the patient’s medications are prescribed and the patient is informed about their use or is subjected to training and is called for control every three months.
Translated with www.DeepL.com/Translator (free version)
In the following controls, a quintuple hormone profile and our special method “Detailed Sperm Germ Leading Cell Analysis” are performed. Do not trust anyone other than ANDROMED who claims to do this because they do not have any knowledge on this subject. We have developed the method first with Mr Arif and then with Mr Tolga. We are now at a more advanced level in embryo trials with spermatids with the elonge staging system. Now we have reached the stage of using it in combination with the genetic screening method. We are more advanced than the real round spermatid periods. Most of our cases have progressed to the highest quality spermatid stages. Now the aim should be to support the study with the highest quality spermatids with the highest quality embryo formation with PGD NGS test and to realise healthy embryo transfer.
Unfortunately, despite all our warnings, embryo transfer continues to be performed randomly and without waiting up to five days for development, with the method of deceiving patients with two pictures without genetic testing. In the precedent decision given by an expert report in the legal struggle made by a conscious family on this issue, the IVF centre was found defective due to the transfer made before the development (partonogenesis) was completed and without genetic verification (PGD NGS).
In each control, the development details are taught and the treatment is changed.
In our country and in the world, a prediction criterion was a great need in azoospermia. Treatments were randomised. In a case where there are no stem cells, a treatment containing FSH will have the opposite effect and make it worse. If some supports such as carob and fig cure are recommended in an unconscious and randomised manner, their condition will also deteriorate and regress.
All natural food approaches should be testosterone boosting. When we use hormone injections that require FSH, testosterone is already decreasing thanks to these injections and spermatids are maturing towards sperm. We do not recommend supports such as Carob, except in cases of extremely serious testosterone increase.
Fig cure support increases prolactin levels. It also lowers testosterone and there is no progress from the stem cell step.
Not every natural wonder herbal support product is randomly recommended for every case with low sperm count, nor should it be!
In fact, the injections produce the hormone production that the patient cannot produce in a healthy and balanced way, but passively. When we say passive, we mean that if the treatment is stopped, it will return again. While the treatment is progressing properly, the right phytotherapy support at the appropriate time and in the right amount creates a synergistic effect in progression and improves the structures we call microenvironment.
In the field of medicine, we call this approach “Holistic Approach” and the medical ethics in this approach “Integrative Medicine”. Because the holistic approach is the basis of our treatment method.
